A spectrophotometric study of the carbohydrate binding siete of concanavalina.

نویسنده

  • H Bucht
چکیده

The carbohydrate binding sites of the jack bean lectin, concanavalin A, were studied spectrophotometrically. A number of ligands were examined for their ability to displace the chromogenic ligand p-nitrophenyl cr-n-mannopyranoside. It is shown that this technique provides a simple and convenient approach for obtaining association constants for a series of displacing ligands. The data can best be interpreted in terms of a single carbohydrate binding site per monomeric unit for the protein in solution.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 249 9  شماره 

صفحات  -

تاریخ انتشار 1973